North Dakota State University

Kendra J. Greenlee

greenlee lab  https://sites.google.com/site/greenleelab/

317 Stevens Hall

701-231-6270

kendra.greenlee@ndsu.edu

Research program:

Insects are remarkably tolerant of exposure to low oxygen, yet the mechanisms by

which they accomplish this are not well understood.  Furthermore, throughout

development, insects undergo huge increases in body size that may require increases

in tracheal system capacity.  In our lab, we use the tobacco hornworm caterpillar,

Manduca sexta, to investigate the respiratory physiology of insects at the molecular,

cellular and whole organism levels.  We use respirometry and synchrotron x-ray

imaging to quantify gas exchange in developing caterpillars.  In addition, we use

immunohistochemistry, flow cytometry and western blotting to assess the function

and expression of proteins involved in the responses to hypoxia, infection and injury.

Reviewed publications:

Goswami , S. , Angkasekwinai P., Shan, M., Greenlee, K. J., Barranco, W. T., Polikepahad, P., Seryshev, A., Song, L.-Z., Redding, D.,

Singh, B., Sur, S., Woodruff, P., Dong, C., Corry, D. B., and Kheradmand, F. (2009).  Divergent functions for airway epithelial matrix

metalloproteinase 7 and retinoic acid in experimental asthma.  Nature Immunology.  doi:10.1038/ni.1719.

Greenlee, K. J., Nebeker, C. and Harrison, J. F. (2007). Body size-independent safety margins for gas exchange across grasshopper

species. Journal of Experimental Biology.  210: 1288-1296.

Greenlee, K. J., Werb, Z., and Kheradmand, F.  Matrix metalloproteinases in lung: Multiple, multifarious, and multifaceted. (2007).

Physiological Reviews. 87: 69-98.

Greenlee, K. J., Corry, D., Engler, D. A., Matsunami, R. K., Tessier, P., Cook, R. G., Werb, Z,, and Kheradmand, F. (2006). Proteomic

identification of in vivo substrates for matrix metalloproteinases 2 and 9 reveals a mechanism for resolution of inflammation. Journal

of Immunology, 177: 7312-7321.

Greenlee, K. J. and Harrison, J. F. (2005).  Respiratory changes throughout ontogeny in the tobacco hornworm caterpillar, Manduca

sexta.  Journal of Experimental Biology. 208: 1385-1392.

Harrison, J. F., Lafreniere, J. J., and Greenlee, K. J. (2005). Ontogeny of tracheal dimensions and gas exchange capacities in the

grasshopper, Schistocerca americana. Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology, 141: 372-380.

Greenlee, K. J. and Harrison, J. F. (2004).  Development of respiratory function in the American locust, Schistocerca americana  I.

Across-instar effects. Journal of Experimental Biology. 204: 497-508.

Greenlee, K. J. and Harrison, J. F. (2004).  Development of respiratory function in the American locust, Schistocerca americana  II.

Within-instar effects. Journal of Experimental Biology. 204: 509-517.

Greenlee, K. J. and Harrison, J. F. (1998). Acid-base and respiratory responses to

hypoxia in the grasshopper, Schistocerca americana. Journal of Experimental Biology. 201: 2843-2855.