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Guodong Liu

Analytical Chemistry – Bioassay, Nanotechnology and Biological Sensing

The emergence of nanotechnology is opening new horizons for the application of nanomaterials in Analytical Chemistry, particularly in biosensing and bioassays. Nanoparticles, nanotubes and nanowires are of considerable interest owing to their unique physical and chemical properties. Such properties (high surface/volume ratios, high electrical conductivity, small entrapment volumes and high stability) offer excellent prospects for chemical and biological sensing and bioassay. The power and scope of such nanomaterials can be greatly enhanced by coupling them with biological recognition reactions and electrical processes (i.e. nanobioelectronics). Research interests in Dr. Liu’s group include:

1. Synthesis of Novel Nanomaterials.
2. Development of Nano-Bioprobes for Biosensors and Bioassays.
3. Development of Biosensor and Bioassay Platforms for Ultrasensitive Detection of Nucleic Acids and Proteins.
4. Electrochemical Sensors and Biosensors for Environmental and Security Monitoring.
5. Development of Portable Clinical Diagnosis Device for Point-of-Care and In-field Applications.

Selected Publications

2010
[1] He, Y.; Zhang, X.; Baloda,M.; GurungA.; Xu,H.; Liu, G.* “Ultrasensitive Nucleic Acid Biosensor Based on Enzyme-Gold Nanoparticle Dual Label and Lateral Flow Strip Biosensor”. Biosensor and Bioelectronics, 2010 (in press)

[2] Fang, Z.; Huang, J.; Lie, P.; Xiao, Z.; Ouyang, C.; Wu, Q.; Wu, Y.; Liu, G.*; Zeng, L. “Lateral Flow Nucleic Acid Biosensor for Cu2+ detection in Aqueous Solution with High Sensitivity and Selectivity” . Chemical Communications 2010 (Accepted)

[3] He, Y.; Zeng,K.; Gurung, A.; Baloda, M.; Xu, H.; Zhang. X.; Liu, G.* Visual Detection of Single-Nucleotide Polymorphism with Hairpin Oligonucleotide-Functionalized Gold Nanoparticles. Analytical Chemistry, 2010, 82 (17), pp 7169–7177.

[4]He, Y.; Zeng, K.; Zhang, X.; Gurung, A.; Baloda, M.; Xu, H.; Liu, G.* “Ultrasensitive electrochemical detection of nucleic acid based on the isothermal strand-displacement polymerase reaction and enzyme dual amplification” Electrochemistry Communications, 2010, 12, 985-988.

[5] Zhao, L.; He, Y.; Mao, X.; Sun, Y.; Zhang, X.; Li, C.; Liu, G.* “Electrochemical assay of active prostate-specific antigen (PSA) using ferrocene-functionalized peptide probes. Electrochemistry Communication 2010, 12, 3, 471-474.

[6] Kawde, A.; Mao, X.; Xu, H.; Zeng, Q.; He, Y.; Liu, G.* “Moving Enzyme-Linked ImmunoSorbent Assay to the Point-of-Care Dry-Reagent Strip Biosensors”. Am. J. Biomed. Sci. 2010, 2(1), 23 - 32

2009
[7] Xu, H.; Mao, X.; Zeng, Q.; Wang,S.; Kawde, A., Liu, G.* Aptamer-Functionalized Gold Nanoparticles as Probes in a Dry-Reagent Strip Biosensor for Protein Analysis. Analytical
Chemistry 2009, 81 (2), 669–675.

[8] Mao, X.; Ma, Y.; Zhang, A.; Zhang. L; Zeng, L.; Liu, G.* Disposable Nucleic Acid Biosensors Based on Gold Nanoparticle Probes and Lateral Flow Strip. Analytical chemistry 2009, 81 (4), 1660–1668.

[9] Zeng, Q.; Mao, X.; Xu, H.; Wang, S.; Liu, G.* Quantitative Immunochromatographic Strip Biosensor for the Detection of Carcinoembryonic Antigen Tumor Biomarker in Human Plasma. Am. J. Biomed. Sci. 2009, 1, 70 – 79.

[10] Mao. X.; Xu. H.; Zeng, Q.; Zeng, L.*; Liu, G.* Molecular Beacon-Functionalized Gold Nanoparticle as Probes in Dry-Reagent Strip Biosensors for DNA analysis. Chemical communications 2009, in press.

[11] Liu, G.*; Mao,X.; Phillips, J. A.; Xu, H.; Tan, W.; Zeng, L. “Aptamer−Nanoparticle Strip Biosensor for Sensitive Detection of Cancer Cells” Analytical Chemistry 2009, 81 (24), 10013-10018.

[12] Prabhulkar, S.; Alwarappan, S.; Liu, G.; Li, C.* “Amperometric micro-immunosensor for the detection of tumor biomarker.” Biosensor and Bioelectronics 2009, 24(12), 3524-3530.

[13] Alwarappan, S., Liu, G., Li, C. “Simultaneous detection of dopamine, ascorbic acid, and uric acid at electrochemically pretreated carbon nanotube biosensors” Nanomedcine, 2009, doi:10.1016/j.nano.2009.06.003

2008
[14] Wang, S.; Zhang, X.; Mao, X.; Zeng, Q.; Xu, H.; Lin, Y.; Chen, W.; Liu, G.* Electrochemical immunoassay of carcinoembryonic antigen based on a lead sulfide nanoparticle label. Nanotechnology 2008, 19, 435501.

[15] Liu, G.*; Wang, J.; Barry, R.; Petersen, C.; Timchalk, C.; Gassman, P. L.; Lin, Y. Nanoparticle-based electrochemical immunosensor for the detection of phosphorylated acetylcholinesterase: an exposure biomarker of organophosphate pesticides and nerve agents. Chem. Eur. J. 2008, 14, 9951-9959.

[16] Mao, X.; Liu, G.*; Wang, S.; Lin, Y.; Zhang, A.; Zhang, L.; Ma, Y. Ultrasensitive electrochemical detection of mRNA using branched DNA amplifiers Electrochemistry Communications, 2008 10, 1847-1850.

[17] Lin, Y.; Wang, J.; Liu, G.; Wu, H.; Wai, C. M.; Lin, Y. A nanoparticle label/immunochromatographic electrochemical biosensor for rapid and sensitive detection of prostate-specific antigen, Biosensors and Bioelectronics, 2008,23, 1659-1665.

[18] Mao, X.; Liu, G.* Nanomaterial based electrochemical DNA biosensors and bioassays. Journal of Biomedical Nanotechnology, 2008, 2008, 4, 419–431.

[19] Mao, X.; Baloda, M.; Gurung, A. S.; Lin, Y.; Liu, G.* Multiplex electrochemical immunoassay using gold nanoparticle probes and immunochromatographic strips, Electrochemistry Communications, 10 (2008) 1636-1640.

[20] Wang, S.; Tan, Y.; Zhao, D.; Liu, G.* Amperometric tyrosinase biosensor based on Fe3O4 nanoparticles–chitosan nanocomposite, Biosensors and Bioelectronics, 23 (2008) 1781-1787.

[21] Lin, Y.; Wang, J.; Liu, G.; Wu, H.; Wai, C. M.; Lin, Y. A nanoparticle label/immunochromatographic electrochemical biosensor for rapid and sensitive detection of prostate-specific antigen, Biosensors and Bioelectronics, 23 (2008) 1659-1665.

[22] Lin, Y.; Liu, G.; Wai, C.M.; Lin, Y. Bioelectrochemical immunoassay of polychlorinated biphenyl. Analytica Chimica Acta, 612 (2008) 23-28.

[23] Wang, J.; Liu, G.; Wu, H.; Lin, Y. Sensitive electrochemical immunoassay for 2, 4, 6-trinitrotoluene based on functionalized silica nanoparticle labels, Analytica Chimica Acta, 610 (2008) 112-118.

[24] Wang, S.; Xu, Q.; Zhang, X.; Liu, G. Sensitive electrochemical determination of calcium dobesilate on the carbon–iron nanoparticle modified glassy carbon electrode, Electrochemistry Communications, 10 (2008) 411-415.

[25] Lee, A.; Liu, G.; Heng, C.; Tan, S.; Lim, T.; Lin, Y. Sensitive Electrochemical Detection of Horseradish Peroxidase at Disposable Screen-Printed Carbon Electrode, Electroanalysis, 20 (2008) 2040-2046.

[26] Wang, S.; Xu, Q.; Liu, G.* Differential Pulse Voltammetric Determination of Uric Acid on Carbon-Coated Iron Nanoparticle Modified Glassy Carbon Electrodes, Electroanalysis, 20 (2008) 1116 -1120.

[27] Wang J.; Liu, G.; Wu, H.; Lin, Y. Quantum-Dot-Based Electrochemical Immunoassay for High-Throughput Screening of the Prostate-Specific Antigen, Small, 4 (2008) 82-86.

2007
[28] Liu G.; Lin, Y. “Electrochemical Quantification of Single-Nucleotide Polymorphisms Using Nanoparticle Probes”, J. Am. Chem. Soc.2007, 129(34); 10394-10401.

[29] Liu G.; Wu, H.; Lin, Y. “Apoferritin Templated Synthesis of Encoded Metallic-Phosphate Nanoparticle Tags”, Anal. Chem. 2007, 79(15); 5614-5619.

[30] Liu G.; Lin, Y., Wai, C. W.; Lin, Y. “Disposable Electrochemical Immunosensor Diagnosis Device Based on Nanoparticle Probe and Immunochromatographic Strip.” Anal. Chem. 2007,79 (20), 7644-7653.

[31] Liu G.*; Wang, J.; Wu, H., Lin, Y.; Lin, Y. “Nanovehicles based bioassay labels.”
Electroanalysis, 2007, 19, 777-785.

[32] Liu, G*, Lin, Y. “Nanomaterial labels in electrochemical immunosensors and immunoassays” Tanlanta, 2007, 74, 308-317.

[33] Timchalk, C.; Campbell, J.; Liu, G.; Lin, Y.; Kousba, A. “Development of a Non-invasive Biomonitoring Approach to Determine Exposure to the Organophosphorus Insecticide Chlorpyrifos in Rat Saliva”, Toxicology and Applied Pharmacology 2007, 219, 217–225.

[34] Lin, Y.; Liu, G.; Wai, C.; Lin, Y. “Magnetic Beads-based Bioelectrochemical Immunoassay of Polycyclic Aromatic Hydrocarbons”, Electrochemistry Communications, 2007, 9(7), 1547-1552.

[35] Wu, H.; Liu, G*; Wang, J.; Lin, Y. “Quantum-dots based electrochemical immunoassay of interleukin-1α”, Electrochemistry Communications, 2007, 9(7), 1573-1577.

2006
[36] Liu, G.; Wang, J.; David, W.; Lin, Y. “Electrochemical proteolytic beacon for detection
of matrix metalloproteinase activities.” J. Am. Chem. Soc., 2006, 128(38), 12382-12383.

[37] Liu, G.; Lin, Y. “Biosensor based on self-assembling acetylcholinesterase on carbon
nanotubes for flow injection/amperometric detection of organophosphate pesticides and nerve agents.” Anal. Chem. .2006,78(3); 835-842.

[38] Wang, J., Liu, G. “Templated one-step synthesis of compositionally encoded nanowire tags”, Anal. Chem. 2006, 78, 2461-2464.

[39] Liu, G.; Wang, J., Lin, Y. “Bioassay label based on apoferritin nanovehicle.” ChemBioChem, 2006, 7(9), 1315-1319.

[40] Liu, G.; Wu, H.; Wang, J., Lin, Y. “Template synthesis of metal phosphate nanaoparticle labels for electrochemical Immunoassay Applications.”Small, 2006, 2(10), 1139-1143.

[41] Liu, G.; Lin, Y. “Biosensor Based on Self-Assembling Acetylcholinesterase on carbon Nanotubes for Flow Injection/Amperometric Detection of Organophosphate Pesticides and Nerve Agents.” Anal. Chem. 2006, 78(3); 835-843.

[42] Liu, G.; Lin, Y. “Amperometric glucose biosensor based on self-assembling glucose oxidase on carbon nanotubes”, Electrochemistry Communications, 2006, 8(2), 251-256.

[43] Liu, G.; Lin, Y., “Layer by layer assembling protein on carbon nanotube template”, Journal of Nanoscience and nanotechnology, 2006, 6, 948–953.

[44] Wang J.; Liu, G.; “Templated One-Step Synthesis of Compositionally Encoded Nanowire Tags”, Anal.Chem. 2006,78, 2461-2464

[45] Wang, J.; Liu, G.; Lin, Y. “Amperometric choline biosensor fabricated through electrostatic assembling of bienzyme/polyelectrolyte hybrid layers on carbon nanotubes”, Analyst, 2006, 131, 477.

2005
[46] Liu, G, Lin, Y. “Electrochemical Sensor for Organophosphate Pesticides and Nerve Agents Using Zirconia Nanoparticles as Selective Sorbents”, Anal. Chem. 2005, 77, 5894-5901.

[47] Liu, G; Lee, T. M.; Wang, J. “Nanocrystal-Based Bioelectronic coding of single nucleotide polymorphisms”, J. Am.Chem. Soc. 2005, 127,38-39.

[48] Munge, B., Liu, G, Collins G., and Joseph Wang, “Multiple Enzyme Layers on Carbon Nanotubes for Ultrasensitive Electrochemical Detection Down to 80 DNA Copies”Anal. Chem. 2005; 77(14); 4662-4666.

[49] Liu, G, Lin, Y. “Enzyme nanoparticle based electronic biosensor”, Chemical Communication, 2005, (27):3481-3483.

[50] Liu, G, Lin, Y. “A Renewable Electrochemical Magnetic Immunosensor Based on Gold Nanoparticle Labels”, Journal of Nanoscience and nanotechnology2005, 5(7):1060-1065.

[51] Liu, G, Lin, Y.,, “Electrochemical Stripping Analysis of Organophosphate Pesticides and Nerve Agents”, Electrochemistry Communication, 7 (2005) 339–343

[52] Liu, G, Lin, Y. “ Carbon nanotube nanoelectrode array for ultrosensitive heavy metal analysis”, Analyst,2005, 130 (7):1098-1101.

[53] Cui, X., Liu, G., Li, Y., Wassana Yantasee, Yuehe Lin, “Electrochemical Sensor Based on Carbon Paste Electrode Modified with K-Hollandite Manganese Dioxide for Detection of Heavy Metals”, Sensor Letters, 2005, 3(1):16-21

[54] Cui, X., Liu, G, Lin, Y. Biosensors Based on Carbon Nanotubes/Nickel Hexacyanoferrate/Glucose Oxidase Nanocomposites”, Nanomedicine, 2005, 1(2):130-135

[55] Liu, G.; Riechers, S., Lin, Y.,“Sensitive electrochemical detection of enzymatically generated thiocholine at carbon nanotube modified glassy carbon electrode ”,Electrochemistry communication, 2005, 7(11), 1163-1169.

[56] Liu, G.; Lin, Y. “Sequential injection/electrochemical immunoassay for quantifying the pesticide metabolite 3,5,6-trichloro-2-pyridinol”, Electrochemistry communication,
2005, 7,1463–1470.

2004
[57] Liu, G.; Wang, J., Kim, K., and Jan, M. “Electrochemical Coding for Multiplexed Immunoassays of Proteins”, Anal. Chem., 2004, 76, 7126-7130

[58] Wang, J.; Liu, G.; Munge, B.; Lin L.; and Zhu, Q., “DNA-based Amplified Bioelectronic Detection and Coding of Proteins”, Angewandte Chemie International Edition 2004, 43, 2158-2161.

[59] Wang, J., Liu, G.;Jan, M. R., “Ultrasensitive Electrical Biosensing of Proteins and DNA: Carbon-Nanotube Derived Amplification of the Recognition and Transduction Events”, J. Am. Chem. Soc. 2004, 126, 3010-3011.

[60] Zhong, T.; Liu, G. “Amperometric Silica Sol-gel Based Immunosensor for Schistosoma Japonicum Antibody Assay.”Analytical Sciences, 2004, 20(3), 537-542.

2003
[61] Wang, J., Liu, G., Merkoçi, A., “Electrochemical Coding Technology For Simultaneous Detection of Multiple DNA Targets”, J. Am. Chem. Soc., 2003, 125, 3214-3215.

[62] Wang, J., Liu, G., Rivas, G. “Encoded Beads for Electrochemical identification”, Anal. Chem., 2003, 75, 17, 4667-4671.

[63] Wang, J., Liu, G., Zhu, Q.“ Indium-tagged Nanorods for Highly Sensitive Electrochemical Detection of DNA Hybridization”. Anal. Chem., 2003, 75(22), 6218-6222.

[64] Wang, J.; Liu, G.; Jan M.; Zhu, Q. “Electrochemical detection of DNA hybridization based on carbon-nanotubes loaded with CdS tags “Electrochemistry Communications, 2003, 5 (12), 1000-1004.

Associate Professor

BS, Hunan Normal University, 1995
MS, Hunan University, Hunan, 1998
PhD, Hunan University, Hunan, 2001
Postdoctoral Fellow, New Mexico State University, 2002-2004
Postdoctoral Fellow, Pacific Northwest National Laboratory, 2004-2006

Office: 158 Dunbar

tel 701-231-8697
fax 701-231-8831

Guodong.Liu@ndsu.edu