Example:

Unrau, J. 1950. Scientific Agric. 30:66-89.

Unrau crossed a euploid wheat cultivar that carried a dominant allele for red-glume color to 17 nullisomic or monosomic genetic stocks of the Chinese Spring variety. He determined that the allele for red-glume color was located on chromosome I. The genetic F₂ ratio of red to white glumed plants was the expected 3:1, except for the cross to the nullisomic stock for chromosome I. When he crossed the euploid cultivar (Federation 41) to the genetic stock that was nullisomic for chromosome I, the F₂ ratio showed a deficiency in the number of white glumed F₂ plants.

Unrau did a cytological examination of these six F₁ plants from the above cross and found all six plants were monosomic.

The F₁ plants were self-fertilized and the F₂ population had 528 red-glumed plants and 38 white glumed plants. The red-glumed F₂ plants were either euploid or monosomic. The white-glumed F₂ plants were nullisomic.

F₁ red-glumed monosomic plant self-fertilized:

The above result was for the F₁ plants self-fertilized that were monosomic for chromosome I and disomic for the other 20 chromosomes. The 528 red-glumed F₂ plants were either disomic or monosomic.