X
Short Formula
Suppose the ratio is X
:X
,
then the proportions are:
X
and X
X
+X
X
+X
Also, the expected numbers for each class would be
(n) X
and (n) X
X
+X
X
+X
Back to our example, we have the ambiguous segregation
ratio 1.9640:1 which can be converted to proportions:
1.9640 and (1)
2.9640 2.9640
Then the expected number of each class are:
(n)1.9640 and
n(1)
2.9640 2.9640
The short formula for a X
of a 3:1 ratio is:
X
= [a
- a
(3)]
= 3.841
3n
Now we know a
is the expected number of the dominant class and a2
is the expected number of the recessive class. Then
substituting the ambiguous expected numbers for each
class gives:

Now we solve for n to find the critical F
family size such that more than r recessives indicates
a 9:7 ratio and less than r recessives indicates a 3:1
ratio.

3.84 = n2 x
(1.96-3)
x 1
(2.9640)
1 3n
3.84 = (1.04)
x n
(2.9640)
3
n = 94.31
We must grow 95 plants to distinguish a 3:1 ratio from
a 9:7 ratio.
Let n=94, we use the ambigous ratio to derive the observed
class and test with a X
to try to distinguish the two ratios.
The dominant class will have the observed number
(95) 1.964 =
62.9~63.
(95) 2.964
The observed number for the recessive class
(95) 1 =
32.
2.964