Double Crossover Detection

Double-crossover Classes

Crossover Frequency Between Lz and Su

Double-crossover Classes

The double-crossover classes show that the Su gene recombined with the Lz and Gl genes. Therefore Su is in the middle, between Lz and Gl.

Result is GllzSu and glLzsu.
Result is GllzSu and glLzsu.
Result is GllzSu and glLzsu.

Result is GllzSu and glLzsu.
Result is GllzSu and glLzsu.
Result is GllzSu and glLzsu.
Result is GllzSu and glLzsu.

If Lz is the middle locus, then the double crossover and least frequent classes would involve an exchange of Lz.

If Gl is in the middle, then double-crossover would result in LzglSu and lgGlsu as the least frequent classes.
Result is GllzSu and glLzsu.
Result is GllzSu and glLzsu.
Result is GllzSu and glLzsu.

If Su is in the middle, then double-crossover would result in GlsuLz and glSulz as the least frequent classes.
Result is GllzSu and glLzsu.
Result is GllzSu and glLzsu.
Result is GllzSu and glLzsu.

We can use three-point testcrosses to map gene order.

We can now summarize the data:

LzSuGl
lzsugl
286
272
Parental types, non-crossover
Lzsugl
lzSuGl
40
33
Single crossover between Lz and Su
LzSugl
lzsuGl
59
44
Single crossover between Su and Gl
LzsuGl
lzSugl
4
2
Double-crossover
Total 740  

The reciprocal products that result from a cross over occur with approximately equal frequency. Deviations are due to sampling.

Copyright 2000©, Ted Helms

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