Testcrosses

McClintlock & Maize Chromosome Analysis Part I

McClintlock & Maize Chromosome Analysis Part II

A-B Translocation in Maize

Microsporegenesis Megasporegenesis

Fertilization Part I

Fertilization Part II

Reciprocal Translocation In Maize - Part I

Reciprocal Translocation In Maize - Part II

Reciprocal Translocation In Maize - Part III

Fertilization - Part II

The anueploid type of pollen can fertilize the seed parent in two ways. One way is for the sperm nucleus with the duplication to fertilize the primary endosperm nucleus while the sperm nucleus with the deficiency fertilizes the embryo. The second way is for the sperm nucleus that is deficient to fertilize the primary endosperm nucleus and the sperm nucleus with the duplication fertilizes the embryo nucleus.

Type I

Type II

Type III

Mapping Genes To The Correct Chromosome

Cytogeneticists can use root-tip smears to identify which chromosome carries the A-B translocation. A-B translocation genetic stocks are developed for each of the ten maize chromosomes. The A-B translocation stock is used as the male parent and carries a dominant marker allele. The female parent is a normal stock with ten A-type chromosomes and is homozygous recessive for the marker locus. A sugary, homozygous recessive corn kernel is evidence that the male gametic nucleus was deficient for the portion of the A chromosome (4B) which contains the su locus. The A-B translocation stock which results in sugary kernals is the chromosome that contains the locus.

Copyright 2000©, Ted Helms

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