Steven (Yue) Qian , Ph.D.
208 Sudro Hall
Office Phone: 701.231.8511
Lab Phone: 701.231.8517
1999-2004 Post-doctoral in Free Radical Biology and Medicine, Lab.
Pharmacology and Chemistry National Institute of Environmental
Health Sciences (NIEHS/NIH), Research Triangle Park, NC.
1994-1999 Ph.D. in Free Radical/Radiation Biology, College of Medicine,
The University of Iowa, Iowa City, IA.
Grants and Fellowships
2009-date PI, National Cancer Institute, NCI (R15CA140833) "Refinement
of LC/ESR/MS for Studying Lipid Free Radicals in Colon
2008-date Pilot Project P.I. NDSU, COBRE and NCRR, NIH (P20 RR015566)
“New strategies for targeting protease in disease”
2008-2009 P.I. NDSU, INBRE
“Identification of Lipid-Derived Radicals Involved in Liver
Proteasome Dysfunction in Ethanol-Fed Rats”
2006-2010 P.I., National Cancer Institute, NIEHS (K22ES-012978)
“Endogenous Radicals, Hepatic Lipid peroxidation, and ALD”
2005-2007 P.I., ND EPScoR and NDSU/College of Pharmacy start-up fund
1999-2004 IRTA Research Fellowship, NIEHS.
2012-date: Tenured Associate Professor, Department of Pharmaceutical Sciences, College of Pharmacy, Nursing, and Allied Sciences, North Dakota State University, Fargo, ND.
2012-date: Guest Researcher/Special Volunteer, Lab. Pharmacology and Chemistry, National Institute of Environmental Health Sciences (NIEHS/NIH), Research Triangle Park, NC NIEHS, NIH.
2006-date: Joint Faculty and Assistant Professor, Cellular and Molecular Biology Program (CMB), North Dakota State University, Fargo, ND.
2008-2012: Joint Faculty Member, Investigator, and Assistant Professor, Center for Protease Research, North Dakota State University, Fargo, ND.
2005-2012: Assistant Professor, Department of Pharmaceutical Sciences, College of Pharmacy, Nursing, and Allied Sciences, North Dakota State University, Fargo, ND.
2004-2007: Guest Researcher/Special Volunteer, Lab. Pharmacology and Chemistry, National Institute of Environmental Health Sciences (NIEHS/NIH), Research Triangle Park, NC NIEHS, NIH
2004-2005: Research Assistant Professor, Division of Drug Delivery and Disposition, School of Pharmacy, The University of North Carolina at Chapel Hill, NC.
1999-2004: IRTA/Visiting Fellow, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC.
1994-1999: Graduate Research Assistant, Free Radical and Radiation Biology Program, College of Medicine, The University of Iowa, Iowa City, IA.
- Free radical chemistry and biology as the related to problems in human health
- Iron and oxygen chemistry as the initiation of free radical biological reaction
- Oxidative stress and toxicology/xenobiotic disposition leading to pathogenesis in disease processes, such as ALD, DILI, neurological, cardiovascular diseases, and cancer.
- COX- and LOX-catalyzed lipid (PUFAs) peroxidation, as the related to cancer and other human health problems
2007-2012 Pharmacodynamics (PSCI 411/611), College of Pharmacy,
NDSU, Fargo ND
2009-2011 Pharmacokinetics (PSCI 470/670), College of Pharmacy,
NDSU, Fargo ND
2010-2012 Cancer Cell Biology (PSCI 796), College of Pharmacy,
NDSU, Fargo ND
2010-2011 Individual Study (PSCI 494), College of Pharmacy,
NDSU, Fargo ND
1996-1998 TAs of Free Radical Bio. Med. (77:222), College of Medicine, UI,
Iowa City, IA
2012 Dean’s Award for Excellence in Research, College of Pharmacy, Nursing, and Allied Sciences, North Dakota State University
2012 Invited Reviewer, NCI Research Grand Study Section, NIH
2012 NDSU Provost Travel and Research Fund
2011 SFRBM Junior Award Committee
2011 Young Investigator Award Committee, 18th SFRBM
2010 Invited Research Grant Reviewer, Florida Department of Health, James/Esther King Biomedical Research Program and Bankhead-Coley Cancer Research Program
2010 Young Investigator Award Committee of 17th SFRBM
2009 NDSU Professional Development Grant
2009 Young Investigator Award Committee, 16th SFRBM
2008 Outstanding Mentor Certificate, NDSU 2008 Summer Undergraduate Research STEM Program
2008 NDSU Professional Development Grant
2008 Young Investigator Award Committee, 15th SFRBM
2007 NDSU Professional Development Grant
2007 Young Investigator Award Committee, 14th SFRBM
2006 Young Investigator Award Committee, 13th SFRBM
2006 NDSU Professional Development Grant
2006 Section Chair, 13th Annual Meeting of SFRBM
2004 NIEHS nominee for Burroughs Wellcome Fund
2003 Research Excellence Award (FARE), National Institutes of Health
2002 Young Investigator Award, International Symposium of EPR Spin Trapping
2001 Young Investigator Award, Free Radical Biology & Medicine (Oxygen) Society
1998 Travel Award, American Society for Photobiology
1997 Travel Awards, University of Iowa
1997 Travel Award, American Society for Photobiology
1996 Young Investigator Awards, Free Radical Biology & Medicine (Oxygen) Society
1996 Travel Awards, University of Iowa
1996 Travel Award, Radiation Research Society
2006-date: American Association for Cancer Research (AACR)
2005-date: American Association of Colleges of Pharmacy (AACP)
1996-date: Society for Free Radical Biology & Medicine (SFRBM)
Wagh A, Qian SY, Law B. Development of biocompatible polymeric nanoparticles for in vivo NIR and FRET imaging. Bioconjugate Chem. In press; 2012.
Qian SY, Xu Y, Gu Y. Combination of Spin-Trapping, LC/ESR and LC/MS Technique in Characterization of PUFA-Derived Free Radials in Lipid Peroxidation. Acta Biophysica Sinica, 28: 355-372; 2012
Senay Simsek, Kimberly Burgess, Kristin L. Whitney, Yan Gu, Steven Y. Qian. Analysis of Deoxynivalenol and Deoxynivalenol-3-glucoside in Wheat. Food Control. In press, 2012.
Purwaha P, Gu Y, Kelavkar U, Kang JX, Law B, Wu E, Qian SY. LC/ESR/MS study of pH-dependent radical generation from 15-LOX catalyzed DPA peroxidation. Free Radic Biol Med. 51: 1461-1470; 2011.
Malik R, Qian SY, Law B. Design and synthesis of a near-infrared fluorescent nanofiber precursor for detecting of cell-secreted urokinase activity. Analytical Biochem. 412: 26-33; 2011.
Nyren-Erickson EK, Haldar MK, Gu Y, Qian SY, Friesner DL, Mallik S. Fluorescent liposomes for differential interactions with glycosaminoglycans. Anal. Chem. 83: 5989-5995; 2011.
Wang F, Bhat K, Doucette M, Zhou S, Gu Y, Law B, Liu X, Wong ET, Kang JX, Hsieh TC, Qian SY, Wu E. Docosahexaenoic acid (DHA) sensitizes brain tumor cells to etoposide-induced apoptosis. Curr. Mol. Med. 11:1-9; 2011.
Zhang W, Phillips K, Wielgus AR, Liu J, Albertini A, Zucca FA, Faust R, Qian SY, Miller DS, Chignell CF, Wilson B, Jackson-Lewis V, Przedborski S, Joset D, Loike J, Hong JS, Sulzer D, Zecca L. Neuromelanin Activates Microglia and Induces Degeneration of Dopaminergic Neurons: Implications for Progression of Parkinson’s Disease. Neurotox Res. 19: 62-72; 2011.
Zou Y, Chang S, Gu Y, Qian SY. Antioxidant activity and phenolic compositions of the extract of lentils (Lens culinaris var. Morton) and its fractions. J. Agr Food Chem. 59: 2268-2276; 2011.
Xiao Y, Gu Y, Purwaha P, Ni K, Law B, Mallik S, Qian SY. Characterization of free radicals formed from COX-catalyzed DGLA peroxidation. Free Radic Biol Med. 50: 1163-1170; 2011.
Berg AK, Yu Q, Haldar MK, Qian SY, Srivastava DK. Solvent-assisted slow conversion of a dithiazole derivative produces a competitive inhibitor of peptide deformylase. B.B.A. 1804: 704-713; 2010.
Subramaniam R, Xiao Y, Li Y, Qian SY, Sun W, Mallik S. Light-mediated and H-bond facilitated liposomal release: The role of lipid head groups in release efficiency. Tetrahedron Letters, 51: 529-532; 2010.
Zhang Q, Yao F, O’Rourke S, Qian SY, Sun C. Angiotensin II enhances GABAB receptor-mediated responses and expression in nucleus tractus solitarii of rats. Am J Physiol Heart Circ Physiol. 297: H1837-H1844; 2009.
Yu Q, Xiao Y, Ni K, Qian SY. Identification and characterization of carbon-centered free radicals formed from lipoxygenase-catalyzed lipid peroxidation of DLA. Chinese J. Analytical Chem. 37:1815-1819, 2009.
Yu Q, Purwaha P, Ni K, Sun C, Mallik S, and Qian SY. Characterization of Novel Radicals from COX-Catalyzed Arachidonic Acid Peroxidation. Free Radic Biol Med., 47: 568-576. 2009.
Syn WK, Yang L, Chiang DJ, Qian Y, Jung Y, Karaca1 G, Choi SS, Witek RP, Omenetti A, Pereira TA, Diehl AM. Genetic differences in oxidative stress and inflammatory responses to diet-induced obesity do not alter liver fibrosis in mice. Liver Int. 29: 1262-1272; 2009.
Shan Z, Yu Q, Guo B, and Qian SY. A combination study of spin-trapping, LC/ESR and LC/MS on carbon-centred radicals formed from lipoxygenase-catalysed peroxidation of eicosapentaenoic acid. Free Radical Research. 43: 1-15; 2009. DOI: http://dx.doi.org/10.1080/10715760802567606, PMID: 19085251.
Yang S, Zhang D, Yang Z, Hu X, Qian S, Liu J, Wilson B, Block M, and Hong JS. Curcumin protects dopaminergic neuron against LPS induced neurotoxicity in primary rat neuron/glia culture. Neurochem Res. 33: 2044-2053; 2008. DOI: http://dx.doi.org/10.1007/s11064-008-9675-z, PMID: 18368483.
Liu J, Qian SY, Guo Q, Jiang J, Waalkes MP, Mason RP, and Kadiiska MB. Cadmium generates reactive oxygen- and carbon-centered radical species in rats: insights from in vivo spin-trapping studies. Free Radic Biol Med. 45: 475-481; 2008. PMID: 18501199, DOI: http://dx.doi.org/10.1016/j.freeradbiomed.2008.04.041.
Yu Q, Shan Z, Ni K, and Qian SY. LC/ESR/MS Study of Spin Trapped Carbon-Centered Radicals Formed From In Vitro Lipoxygenase-Catalyzed Peroxidation of -Linolenic Acid. Free Radical Research. 42: 442-455; 2008. DOI: http://dx.doi.org/10.1080/10715760802085344. PMID: 18484409;
Yang Z, Yang S, Hong JS, Qian SY, Kadiiska MB, Tennant RW, Waalkes MP, and Liu J. Cadmium-induced toxicity in rat primary mid-brain neuro-glia cultures: role of oxidative stress from microglia. Toxicol. Sci. 98: 488-494; 2007. PMID: 17483498, DOI: http://dx.doi.org/10.1093/toxsci/kfm106.
Shan X, Chi L, Ke Y, Luo C, Qian S, Gozal D, Liu R. Manganese superoxide dismutase protects mouse cortical neurons from chronic intermittent hypoxia-mediated oxidative damage. Neurobiol Dis. 28: 206-215; 2007. PMID: 17719231, DOI: http://dx.doi.org/10.1016/j.nbd.2007.07.013.
Dyers JD, Que (Qian, misspell) SY, VanDerveer D, and Bu XR. Synthesis and structures of new salen complexes with bulky groups. Inorg. Chim. Acta. 359: 197-203; 2006.
Qian SY, Kadiiska MB, Guo Q, Liu J, and Mason RP. A novel protocol to identify and quantify all spin trapped free radicals from in vitro/in vivo interaction of HO• and DMSO: LC/ESR, LC/MS, and dual spin-trapping combinations. Free Radic. Biol. Med. 38: 125-135; 2005. PMID: 15589381, DOI: http://dx.doi.org/10.1016/j.freeradbiomed.2004.09.024.
Guo Q, Gao G, Qian SY, and Mason RP. Novel identification of a sulfur-centered radical-derived DMPO nitrone adduct formed from the oxidation of dithiothreitol by LC/ELISA, LC/ESI-MS and LC/MS/MS. Chem. Res. Toxicol. 17: 1481-1490; 2004. PMID: 15540946, DOI: http://dx.doi.org/10.1021/tx049837o.
Colon ML, Qian SY, Vanderveer D, and Bu XR. Chiral bimetallic complexes from chiral salen metal complexes and mercury (II) halides and acetates. Inorg. Chim. Acta. 357: 83-88; 2004.
Towner RA, Qian SY, Kadiiska MB, and Mason RP. In vivo identification of aflatoxin-induced free radical in rat bile. Free Radic. Biol. Med. 35: 1330-1340; 2003. PMID: 14607532, DOI: http://dx.doi.org/10.1016/j.freeradbiomed.2003.08.002.
Qian SY, Guo Q, Mason RP. Identification of spin trapped carbon-centered radicals in soybean lipoxygenase-dependent peroxidations of ω-3 polyunsaturated fatty acids by LC/ESR, LC/MS, and Tandem MS. Free Radic. Biol. Med. 35: 33-44; 2003. PMID: 12826254, DOI: http://dx.doi.org/10.1016/S0891-5849(03)00217-X.
Guo Q, Qian SY, and Mason RP. Separation and identification of DMPO adducts of oxygen-centered radicals formed from organic hydroperoxides by HPLC-ESR, ESI-MS and MS/MS. J. Am. Soc. Mass Spectr. 14: 862-871; 2003. DOI: http://dx.doi.org/10.1016/S1044-0305(03)00336-2, PMID: 12892910.
Qian SY, Yue GH, Tomer KB, and Mason RP. Identification of all classes of spin-trapped carbon-centered radicals in soybean lipoxygenase-dependent lipid peroxidations of ω-6 PURA via LC/ESR, LC/MS and MS/MS. Free Radic. Biol. Med. 34: 1017-1028; 2003. PMID: 12684086, DOI: http://dx.doi.org/10.1016/S0891-5849(03)00031-5.
Qian SY, Tomer KB, Yue GH, Guo Q, Kadiiska MB, and Mason RP. Characterization of the initial carbon-centered pentadienyl radical and subsequent radicals in lipid peroxidation: Identification via LC/ESR and MS. Free Radic. Biol. Med. 33: 998-1009; 2002. PMID: 12361809, DOI: http://dx.doi.org/10.1016/S0891-5849(02)00992-9.
Guo Q, Corbett JT, Yue GH, Fann YC, Qian SY, Tomer KB, and Mason RP. Electron spin resonance investigation of semiquinone radicals formed from the reaction of ubiquinone 0 with human oxyhemoglobin. J. Biol. Chem. 277: 6104-6110; 2002. PMID: 11748217, DOI: http://dx.doi.org/10.1074/jbc.M106395200.
Qian SY, Chen YR, Deterding LJ, Fann YC, Chignell CF, Tomer KB, and Mason RP. Identification of protein-derived tyrosyl radical in the reaction of cytochrome c and hydrogen peroxide: characterization by ESR spin-trapping, HPLC and MS. Biochem. J. 363: 281-288; 2002. PMID: 11931655.
Liang S, Derveer DV, Qian SY, Sturgeon B, and Bu XR. A chiral salen vanadyl complex with two significantly different configurations in solid state: synthesis, characterization, and molecular structure. Polyhedron. 21: 2021-2025; 2002.
Wang HP, Qian SY, Schafer FQ, Domann FE, Oberley LW, and Buettner GR. Phospholipid hydroperoxide glutathione peroxidase protects against singlet oxygen-induced cell damage of photodynamic therapy. Free Radic. Biol. Med. 30: 825-835; 2001. PMID: 11295525, DOI: http://dx.doi.org/10.1016/S0891-5849(01)00469-5.
Qian SY, Wang HP, Schafer FQ, and Buettner GR. EPR detection of lipid-derived free radicals from PUFAs, LDL, and cell oxidations. Free Radic. Biol. Med. 29: 568-579; 2000. PMID: 11025200, DIO: http://dx.doi.org/10.1016/S0891-5849(00)00407-X.
Schafer FQ, Qian SY.; and Buettner GR. Iron and free radical oxidations in cell membranes. cell. Mol. Biol. 46: 657-662; 2000. PMID: 10872752.
Qian SY, and Buettner GR. Iron and dioxygen chemistry is an important route to initiation of biological free radical oxidations. Free Radic. Biol. Med. 26: 1447-1456; 1999. PMID: 10401608, DOI: http://dx.doi.org/10.1016/S0891-5849(99)00002-7.
Liu NF, Chen RX, Zhang LR, and Qian, Y. Detection of hydroxyl radicals in the oxidation of LDL by ESR spin trapping technique. Chinese J. Pathophysiology. 10: 631-634; 1994.
Yan H, Chen HL, Qian Y, and Tang WX. The first study on 2’,5’-dideoxynucleosidyl radicals formed from the anaerobic photolysis of 2’,5’-dideoxynucleosidylcobalamines by the ESR spin-trapping technique. J. Inorg. Biochem. 56: 265-271; 1994.
Xu H, Xu Z, Li MX, Li BL, Qian Y, You XZ, Zhu DL, and Yu YP. Study on basicity of axial ligands effects on monomeric dioxygen adduct of cobalt(II)-schiff base complex by ESR method. J. Inorg. Chem. 9: 331-335; 1993.
Xu JH, He JP, and Qian Y. Photoinduced coupling reaction of benzotriazole with aromatic hydrocarbons sensitized by 9, 10-dicyanoanthracene (DCA) via an electron-transfer mechanism. J. Chem. Soc., Chem. Comm. 10: 714-715; 1991.
Fen R, Qian Y, Xu JY, Wang SW, Li HM, and Shi Q. Therapeutic ultrasound cavitation and its free radical ESR determination. Acta Acustica. 16: 306-312; 1991.
Chen HL, Qian Y, Chen GJ, and Tang WX. Study of coenzyme B12 model compounds: identification of alkyl radicals in RCo(Salen)L solution by spin trapping-ESR techniques. Chinese Science Bulletin. 36: 42-46; 1991.
Fen R, Qian Y, Xu JY, Shi Q, Wang SW, and Li HM. Therapeutic ultrasound cavitation and its free radical ESR determination. Chinese J. Acoustics. 10: 131-138; 1991.
Chen HL, Qian Y, Chen GJ, and Tang WX. Study of coenzyme B12 model compounds: EPR identification of alkyl radicals in RCo(Salen)L solution. Chinese Science Bulletin. 14: 1070-1073; 1990.
Shi Q, Qian Y, Fen R, Liu H, Xu JY, and Li HM. Spectrofluorimetry study of free radicals produced by simulated therapeutic ultrasound. Acta Biophysica Sinica. 6: 485-489; 1990.
Sun SH, Meng QJ, You XZ, Qian Y, and Bu XR. Electrochemistry of RCo3(CO)9 and ESR Spectra of RCo3(CO)9•-. Chinese J. Magnetic Resonance. 6: 137-142; 1989.
Gan LR, Wu YY, Li H, Hu YF, and Qian Y. Study on some bis-schiff bases from isophthalic aldehyde and their copper (II) complexes. J. Inorg. Chem. 5: 81-89; 1989.
Meng QJ, Bu XR, Sun SH, Qian Y, and You XZ. ESR studies on iminato-VO(II) complexes. J. Inorg. Chem. 5: 40-45; 1989.
Yang SY, Ye F, Qian Y, Gao JY, and Liu YC. ESR studies of -tocopherol model-chromanoxyl radical. Acta Biophysica Sinica. 5: 349-353; 1989.
Qian Y. Sonochemistry: historical development and modern aspects (Translated Review). J. Jian Teachers College. 6: 75-88; 1988.
Li HM, Qian Y, Tang JH, and Fen R. Preliminary research on cavitation on medical ultrasound. J. Jian Teachers College. 5: 1-4; 1988.