PLSC 368: Lecture 11

PRINCIPLES OF GRAFTING AND BUDDING

I. WHY GRAFTING AND BUDDING?

    A. Perpetuation of Clones
            -Cultivars that can not be maintained by cuttings, layers, divisions, etc.
                i.e. Eucalyptus, spruce, oak
            -Heterozygous woody plants in adult growth phase
                i.e. juvenility delays fruiting when seedling-grown
            -Maintaining certain lines in breeding


    B. Benefits of Certain Rootstocks
            -Used when plants are selected for ornamental or fruit characteristics not for root adaptability
                plants are then grafted on to rootstocks adapted to poor soil conditions
            -Used to control plant size
                i.e. East malling apple rootstocks dwarf grafted plants----> dwarf apple
            -Use of disease resistant rootstocks
                i.e. Greenhouse cucumber grafted onto rootstocks resistant to Fusarium, verticillium wilts

            -Use of interstocks
                -desirable interstock combined with disease resistant scion and adapted rootstock
                    i.e. rubber tree
                -to reduce vigor of scion wood
                    i.e. "Malling 9" interstock---------> size of tree reduced

    C. Changing Cultivars of Established Plants (topworking)
            -When a new cultivar needs to be grown on an established orchard

            -When a different cultivar is needed for pollination in a single-cultivar orchard


            -Inducing fruit set on pistillate plants of a dioecious species


            -Producing fruits of different cultivars on the same plant
                i.e. apple, pear, citrus, apricot, peach, etc.
            -Producing different color flowers on the same tree
                i.e. Rose


    D. Faster Maturity in Seedling Selections

            -Reduces time required to reach a reproductive maturity
                  ex.  a seedling plant takes 5-10 years to bloom
                        but the same seedling grafted on a rootstock blooms in 2-3 years
            -Increases vigor in seedling-grown plant when grafted
                i.e. Chinese lilac hybrid (Syringa laciniata x S. vulgaris) is grafted on S. amurensis japonica to give vigor and flowering

    E. Combining Special Forms
            -Unusual type of growth habit
                i.e. weeping mulberry

            -Cactus grafting

    F. Repairing Damaged Trees
            -Approach graft
            -Bridge graft

    G. Study of Virus Diseases
            -Scion or buds of suspected plants are grafted on to an indicator plant for symptom development

                        examples
                            -Chrysanthemum
                            -Cherries:
                                    Shirofugen flowering cherry (Prunus serrulata)
                                       -used as an indicator plant for virus detection in peach, almond, apricot, plum

II. GRAFT UNION

    The sequence of healing events after grafting

        1) Lining up of vascular cambiums
            -the cambium layers of scion and stock must be lined up
        2) Formation of necrotic material from cells on the wound site----- healing response
        3) Callus bridge formation
            -parenchyma cell mass produced from the cambial layers fills up the spaces between scion and stock
        4) Cambium formation
            -certain cells of the callus line up forming a cambium layer connecting the cambiums of both the scion and the stock
        5) Vascular tissue formation
            -xylem inside
            -ploem outside