Seminar Abstract
April 16, 2003:
"Direct Recording of Fluorescence Decay in Molecules"
Dr. Greg Gillispie
President, Dakota Technologies, Inc.
Department of Chemistry
North Dakota State University
Measurement of fluorescence light emission provides the basis for many
analysis techniques in the life and physical sciences and for environmental
monitoring. The fluorescence spectrum, i.e., a plot of intensity as a
function of wavelength, is the usual data format. The fluorescence decay
represents an independent type of "spectrum," wherein the intensity is
measured as a function of the time interval after an excitation pulse. The
term fluorescence lifetime refers to the characteristic time constant (or
equivalently, the inverse of the rate constant) for the decay. Fluorescence
decay/lifetime data are extremely valuable because they directly reflect a
molecular property. For example, the intensity of the excitation source can
drift considerably without affecting the accuracy or precision of the
fluorescence lifetime. My presentation will introduce you to the two
traditional means to study fluorescence decay, before I describe DTI's
approach, which can generate high quality data 100-1000 times faster. Data
recently collected at DTI will give you a feel for the important hardware
and data analysis issues. I will also comment on some of the economic issues
that are important for commercialization of the technology.
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