Central Dogma of Molecular Genetics Restriction-Modification Systems of Bacteria Cloning Vectors cDNA Cloning Clone Library Screening DNA Sequencing Southern and Northern Analysis Exons and Introns Polymerase Chain Reaction (or PCR) Study Questions Cloning and Molecular Analysis of Genes WWW Links Genetic Topics

Study Questions What are the basic features of the restriction-modification system found in bacteria? Describe the features that distinguish plasmid, phage, lambda, BAC and YAC cloning vectors. Describe the essential steps for obtaining a clone of a specific fragment of DNA. What are the different types of information that you would obtain from performing a Southern or Northern hybridization. How would you describe the relationship between exons, introns, the gene and its mRNA? Describe the essential steps of the polymerase chain reaction. Copyright © 1997. Phillip McClean