Introduction

Agrobacterium
-mediated Transformation

Introduction of
Foreign Genes via
Agrobacterium-
mediated Transformation

Analyzing Gene Expression
With Transgenic Plants

Course Topics

Course Home
Page

Introduction

What is plant transformation and transgenic plants. The term transformation is used exactly as described by Griffith in 1929.

Transformation - changing the phenotype of an organism by the addition of foreign DNA to is genome

And transgenic plants are generated by introducting foreign DNA into a plant tissue and regnerating plants contain the foreign DNA.

Types of Plant Transformation

  • Agrobacterium-mediated
    • A. tumefaciens
    • A. rhizogenes
  • Uptake of naked DNA
    • electroporation
    • PEG-mediated
    • particle gun

Copyright © 1998. Phillip McClean