Van Es 132a
701 231 8530
- Ph.D. in Microbiology from the University of Ulster (2002).
My primary teaching interest is infectious disease; a subject that is never dull. ‘New’ pathogens emerge, ‘old’ pathogens reveal their secrets, drugs are developed (closely followed by resistance), and the struggle between microorganisms and humanity goes on. Having previously taught Pathogenic Microbiology (MICR460/660) and Infectious Disease Pathogenesis (MICR 450/650), I began teaching Clinical Parasitology (MICR 463/663) in spring 2013. Continuing the theme of infectious disease, Dr. Schuh and I combine our respective graduate courses in Immunology of Chronic Infections (MICR 770) and Advanced Pathogenic Microbiology (MICR 762) to explore infectious disease from the host and pathogen perspectives. We focus on the three big infectious diseases: HIV/AIDS, tuberculosis, and malaria, taking time to discuss social impacts in addition to basic science. Other classes I teach include Scientific Integrity (BIOC 720) and Professional Development (MICR 794).
My primary research interests center around the protozoan parasite, Cryptosporidium. Following a Ph.D. with a bacteriology focus, I found myself working on a European Union funded risk assessment of Cryptosporidium (a eukaryote!) in food and water. Cryptosporidium proved to be equal parts fascinating and frustrating and I wasn’t sure if I was happy or sad when the project ended. Time obviously helped me forget the frustrations because two years later, as I was setting up my lab at NDSU, I decided to make Cryptosporidium a major focus.
Cryptosporidium is a waterborne parasite that causes cryptosporidiosis, a disease affecting humans and animals for which there is no effective treatment. Cryptosporidiosis is especially severe in immunocompromised persons, such as those with AIDS, where diarrhea can become chronic and life threatening. North Dakota, Minnesota and Wisconsin have among the highest incidences of human cryptosporidiosis in the United States.
The following are some Cryptosporidium projects that my lab is currently working on:
- We are working with Drs. Eakalak Khan (Civil Engineering, NDSU) and Mark Clark (Biological Sciences, NDSU) to study the source, fate and transport of Cryptosporidium affecting surface water in the region. This project is funded by USDA-NRI.
- Supported by NIH, through the NDSU Center for Protease Research, we are studying mechanisms of motility, attachment, and invasion in Cryptosporidium.
- As an obligate parasite, the selective pressures on Cryptosporidium are largely provided by the host. Determining how Cryptosporidium adapts to and coevolves with its host has implications for our understanding of parasite biology and pathogen emergence. Working primarily with Dr. Mark Clark, we have been using small mammals as a model for understanding how factors such as host population density and diversity affect Cryptosporidium infection.
- In addition to our focus on Cryptosporidium, we work with Dr. Eakalak Khan on a number of environmental microbiology projects; including those addressing atrazine removal by bacteria, enhancement of bioremediation through entrapment of bacteria, and the effect of nanoparticles on bacterial viability. This work is supported by NSF.
- Kasi, M., Wadhawan, T., McEvoy, J., Padmanabhan, G., Khan, E., 2013a. Effect of carbon source during enrichment on BTEX degradation by anaerobic mixed bacterial cultures. Biodegradation 24, 279-293.
- Kasi, M., Wadhawan, T., Simsek, H., McEvoy, J., Padmanabhan, G., Sletten, D., Khan, E., 2013b. Enricher reactor - Permeable reactive biobarrier approach for removing a mixture of contaminants with substrate interactions. Bioresour. Technol. 146, 336-344.
- Kváč, M., Kestřánová, M., Pinková, M., Květoňová, D., Kalinová, J., Wagnerová, P., Kotková, M., Vítovec, J., Ditrich, O., McEvoy, J., Stenger, B., Sak, B., 2013a. Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa). Vet. Parasitol. 191, 218-227.
- Kváč, M., McEvoy, J., Loudová, M., Stenger, B., Sak, B., Květoňová, D., Ditrich, O., Rašková, V., Moriarty, E., Rost, M., Macholán, M., Piálek, J., 2013b. Coevolution of Cryptosporidium tyzzeri and the house mouse (Mus musculus). Int. J. Parasitol. 43, 805-817.
- Kváč, M., Ondrackova, Z., Květoňová, D., McEvoy, J., Vitovec, J., Rost, M., Sak, B., 2013c. The Lesser Egyptian Gerbil (Gerbillus gerbillus) is a suitable host for the long-term propagation of Cryptosporidium andersoni. Exp. Parasitol. 134, 438-442.
- Kváč, M., Sakova, K., Květoňová, D., Kicia, M., Wesolowska, M., McEvoy, J., Sak, B., 2013d. Gastroenteritis caused by the Cryptosporidium hedgehog genotype in an immunocompetent man. J. Clin. Microbiol.
- Nemejc, K., Sak, B., Květoňová, D., Hanzal, V., Janiszewski, P., Forejtek, P., Rajsky, D., Ravaszova, P., McEvoy, J., Kváč, M., 2013. Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe. Vet. Parasitol. 197, 504-508.
- Rašková, V., Květoňová, D., Sak, B., McEvoy, J., Edwinson, A., Stenger, B., Kváč, M., 2013. Human cryptosporidiosis caused by Cryptosporidium tyzzeri and C. parvum isolates presumably transmitted from wild mice. J. Clin. Microbiol. 51, 360-362.
- Herges, G.R., Widmer, G., Clark, M.E., Khan, E., Giddings, C.W., Brewer, M., McEvoy, J.M., 2012. Evidence that Cryptosporidium parvum populations are panmictic and unstructured in the Upper Midwest of the United States. Appl. Environ. Microbiol. 78, 8096-8101.
- Kváč, M., Kestřánová, M., Květoňová, D., Kotková, M., Ortega, Y., McEvoy, J., Sak, B., 2012. Cryptosporidium tyzzeri and Cryptosporidium muris originated from wild West-European house mice (Mus musculus domesticus) and East-European house mice (Mus musculus musculus) are non-infectious for pigs. Exp. Parasitol. 131, 107-110.
- Kasi, M., McEvoy, J., Padmanabhan, G., Khan, E., 2011. Groundwater remediation using an enricher reactor-permeable reactive biobarrier for periodically absent contaminants. Water Environ. Res. 83, 603-612.
- Pramanik, S., Khanna, R., Katti, K., McEvoy, J., Khan, E., 2011a. Effects of entrapment on nucleic acid content, cell morphology, cell surface property, and stress of pure cultures commonly found in biological wastewater treatment. Appl. Microbiol. Biotechnol. 92, 407-418.
- Pramanik, S., McEvoy, J., Siripattanakul, S., Khan, E., 2011b. Effects of cell entrapment on nucleic acid content and microbial diversity of mixed cultures in biological wastewater treatment. Bioresour. Technol. 102, 3176-3183.
- Wadhawan, T., Maruska, Z.B., Siripattanakul, S., Hill, C.B., Gupta, A., Pruss, B.M., McEvoy, J.M., Khan, E., 2011. A new method to determine initial viability of entrapped cells using fluorescent nucleic acid staining. Bioresour. Technol. 102, 1622-1627.