Biofilm can be defined as single species or multispecies bacterial communities that grow on solid surfaces or solid-liquid interfaces. An extracellular matrix of polymeric substances, an important phenotypic characteristic of the maturation phase, makes biofilm a thousand times more antibiotic resistant than planktonic bacteria. Because of this, biofilm research has increased rapidly over the past 15 years. From past studies, quorum sensing, which is defined as the cell-to-cell communication of bacteria, has already been established as a target mechanism against biofilms. According to a high-throughput experiment in our lab, several two-component signal transduction systems (2CSTS) may also exert significant effects on biofilm formation. In my project, we will propose 2CSTSs for the development of novel prevention and treatment techniques for biofilm-associated infectious diseases by identifying the particular 2CSTSs that are expressed during the early phases of biofilm formation (prevention), as well as the particular regulators that are expressed at the surface of the fully developed three-dimensional structure (treatment). We will focus on the temporal and spatial expression of selected 2CSTSs along with the global regulator FlhD/FlhC. We will study the spatial and temporal expression from those promoters using novel fluorescence microscopy techniques and the fluorescence probes, the spatial as well as the temporal expression from those promoters will be studied.