High Performance Liquid Chromatography (HPLC)

HPLCHigh Performance Liquid Chromatography (HPLC) is an analytical procedure used for chromatographic separation, identification and quantification of compounds that absorb in the UV-VIS range (190 – 950 nm). The Agilent 1100 Series HPLC consists of a Diode-Array Detector (DAD), Fluorescence Detector (FLD), Fraction Collector, Quaternary Pump, Autosampler, Thermostatted Column Compartment, and Vacuum Degasser.

Quaternary Pump
Flow Range: 0.001 – 10 mL/min (settable with degasser), for gradient analysis
(Column ID: 3 - 9.4mm)

Vacuum Degasser
Flow Rate: up to 10 mL/min
Internal Volume: 12 mL

Thermostatted Column Compartment
Temperature Range: 10°C below ambient to 80°C

Standard Autosampler
Injection Range: 0.1 – 100 µL in 0.1 µL increments
Sample capacity: 100 x 2-mL vials
Carry-over: typically <0.1%, <0.05% with external needle cleaning

Fraction Collector
Flow Rate: up to 10 mL/min
Delay Volume: 37 µL

Diode-Array Detector (DAD)
For multi-λ and spectral analysis
Detector type: 1024-element diode array
Light Source: Deuterium and tungsten lamp
Wavelength range: 190 – 950 nm

Fluorescence Detector (FLD)
For multi-signal detection and online fluorescence spectra
Light Source: Xenon flash lamp
Excitation range: 200 – 700 nm
Emission range: 200 – 900 nm