John McEvoy

Professor and Interim Head

Van Es 150
701 231 8530
john.mcevoy@ndsu.edu

Education

  • Ph.D. in Microbiology from the University of Ulster (2002).

Teaching Interests

My teaching interest is infectious disease, a subject that is never dull. New pathogens emerge, old pathogens reveal their secrets, drugs are developed (closely followed by resistance), and the struggle between microorganisms and humanity goes on. I have previously taught Pathogenic Microbiology (MICR460/660) and Infectious Disease Pathogenesis (MICR 450/650). I currently teach Clinical Parasitology (MICR 463/663, which is offered in the spring.

Research Interests

The major focus of my lab is Cryptosporidium, a protozoan parasite that causes cryptosporidiosis, a potentially life threatening diarrheal disease affecting humans and animals. Cryptosporidium is the primary cause of toddler death from infectious diarrheal disease in underdeveloped countries, a cause of severe diarrheal disease and death in immunodeficient and malnourished adults, and a major cause of waterborne disease in the United States and worldwide. Drugs to treat Cryptosporidium infections are not effective and there is no vaccine.

Cryptosporidium research in my lab is focused on three main areas:

  • Host-parasite coevolution
  • Transmission dynamics in humans, animals, and the environment.
  • Molecular mechanisms of parasite development and host interactions.

In collaboration with Dr. Eakalak Khan in the Department of Civil and Environmental Engineering at NDSU, my lab also has an environmental microbiology focus, with research on biodegradation of agricultural pollutants, water and wastewater treatment processes, and the impact of nanomaterial pollutants on environmental microorganisms.

Publications

2016

  • Edwinson, A., Widmer, G., McEvoy, J., 2016. Glycoproteins and Gal-GalNAc cause Cryptosporidium to switch from an invasive sporozoite to a replicative trophozoite. Int. J. Parasitol. 46, 67-74. PDF
  • Chelladurai, J.J., Clark, M.E., Kvac, M., Holubova, N., Khan, E., Stenger, B.L., Giddings, C.W., McEvoy, J., 2016. Cryptosporidium galli and novel Cryptosporidium avian genotype VI in North American red-winged blackbirds (Agelaius phoeniceus). Parasitol. Res. PDF
  • Kváč, M., Havrdová, N., Hlasková, L., Danková, T., Kanděra, J., Jezková, J., Vítovec, J., Sak, B., Ortega, Y., Xiao, L., Modrý, D., Chelladurai, J.R., Prantlová, V., McEvoy, J., 2016. Cryptosporidium proliferans n. sp. (Apicomplexa: Cryptosporidiidae): molecular and biological evidence of cryptic species within gastric Cryptosporidium of mammals. PloS one 11, e0147090. PDF
  • Wagnerova, P., Sak, B., McEvoy, J., Rost, M., Sherwood, D., Holcomb, K., Kvac, M., 2016. Cryptosporidium parvum and Enterocytozoon bieneusi in American Mustangs and Chincoteague ponies. Exp. Parasitol. PDF
  • Kotková, M., Němejc, K., Sak, B., Hanzal, V., Květoňová, D., Hlásková, L., Čondlová, S., McEvoy, J., Kváč, M., 2016. Cryptosporidium ubiquitum, C. muris and Cryptosporidium deer genotype in wild cervids and caprines in the Czech Republic. Folia Parasitol. (Praha). 63. PDF
  • Chaithawiwat, K., Vangnai, A., McEvoy, J.M., Pruess, B., Krajangpan, S., Khan, E., 2016. Impact of nanoscale zero valent iron on bacteria is growth phase dependent. Chemosphere 144, 352-359. PDF

2015

  • Wagnerova, P., Sak, B., McEvoy, J., Rost, M., Matysiak, A.P., Jezkova, J., Kvac, M., 2015. Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland. Parasitol. Res. 114, 1619-1624. PDF
  • Thuptimdang, P., Limpiyakorn, T., McEvoy, J., Pruss, B.M., Khan, E., 2015. Effect of silver nanoparticles on Pseudomonas putida biofilms at different stages of maturity. J. Hazard. Mater. 290, 127-133. PDF
  • Stenger, B.L., Clark, M.E., Kvac, M., Khan, E., Giddings, C.W., Prediger, J., McEvoy, J.M., 2015. North American tree squirrels and ground squirrels with overlapping ranges host different Cryptosporidium species and genotypes. Infect. Genet. Evol. 36, 287-293. PDF
  • Stenger, B.L., Clark, M.E., Kváč, M., Khan, E., Giddings, C.W., Dyer, N.W., Schultz, J.L., McEvoy, J.M., 2015. Highly divergent 18S rRNA gene paralogs in a Cryptosporidium genotype from eastern chipmunks (Tamias striatus). Infect. Genet. Evol. 32, 113-123. PDF
  • Mhuantong, W., Wongwilaiwalin, S., Laothanachareon, T., Eurwilaichitr, L., Tangphatsornruang, S., Boonchayaanant, B., Limpiyakorn, T., Pattaragulwanit, K., Punmatharith, T., McEvoy, J., Khan, E., Rachakornkij, M., Champreda, V., 2015. Survey of microbial diversity in flood areas during Thailand 2011 food crisis using high-throughput tagged amplicon pyrosequencing. PloS one 10, e0128043. PDF
  • Le, T., McEvoy, J., Khan, E., 2015. The effect of single-walled carbon nanotubes on Escherichia coli: multiple indicators of viability. J Nanopart Res 17, 1-9. PDF
  • Laatamna, A.E., Wagnerová, P., Sak, B., Květoňová, D., Xiao, L., Rost, M., McEvoy, J., Saadi, A.R., Aissi, M., Kváč, M., 2015. Microsporidia and Cryptosporidium in horses and donkeys in Algeria: Detection of a novel Cryptosporidium hominis subtype family (Ik) in a horse. Vet. Parasitol. 208, 135-142. PDF
  • Kváč, M., Tomanová, V., Samková, E., Koubová, J., Kotková, M., Hlasková, L., McEvoy, J., Sak, B., 2015. Encephalitozoon cuniculi in raw cow's milk remains infectious after pasteurization. Foodborne pathogens and disease. PDF
  • Kváč, M., Horická, A., Sak, B., Prediger, J., Salat, J., Sirmarová, J., Bartonická, T., Clark, M., Chelladurai, J.R., Gillam, E., McEvoy, J., 2015. Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic. Parasitol. Res. PDF
  • Guo, Y., Cebelinski, E., Matusevich, C., Alderisio, K.A., Lebbad, M., McEvoy, J., Roellig, D.M., Yang, C., Feng, Y., Xiao, L., 2015. Subtyping novel zoonotic pathogen Cryptosporidium chipmunk genotype I. J. Clin. Microbiol. 53, 1648-1654. PDF

2014

  • Wadhawan, T., Simsek, H., Kasi, M., Knutson, K., Prüβ, B., McEvoy, J., Khan, E., 2014. Dissolved organic nitrogen and its biodegradable portion in a water treatment plant with ozone oxidation. Water Res. 54, 318-326. PDF
  • Němejc, K., Sak, B., Květoňová, D., Hanzal, V., Janiszewski, P., Forejtek, P., Rajsky, D., Kotková, M., Ravaszová, P., McEvoy, J., Kváč, M., 2014. Prevalence and diversity of Encephalitozoon spp. and Enterocytozoon bieneusi in wild boars (Sus scrofa) in Central Europe. Parasitol. Res. 113, 761-767. PDF
  • Kváč, M., Sakova, K., Květoňová, D., Kicia, M., Wesolowska, M., McEvoy, J., Sak, B., 2014. Gastroenteritis caused by the Cryptosporidium hedgehog genotype in an immunocompetent man. J. Clin. Microbiol. 52, 347-349. PDF
  • Kváč, M., Němejc, K., Kestřánová, M., Květoňová, D., Wagnerová, P., Kotková, P., Rost, M., Samková, E., McEvoy, J., Sak, B., 2014. Age related susceptibility of pigs to Cryptosporidium scrofarum infection. Vet. Parasitol. 202, 330-334. PDF
  • Kváč, M., McEvoy, J., Stenger, B., Clark, M., 2014. Cryptosporidiosis in other vertebrates, in: Cacciò, S.M., Widmer, G. (Eds.), Cryptosporidium: parasite and disease. Springer Vienna, Vienna, Austria, pp. 237-323.
  • Kváč, M., Hofmannová, L., Hlásková, L., Květoňová, D., Vítovec, J., McEvoy, J., Sak, B., 2014. Cryptosporidium erinacei n. sp. (Apicomplexa: Cryptosporidiidae) in hedgehogs. Vet. Parasitol. 201, 9-17. PDF

2013

  • Rašková, V., Květoňová, D., Sak, B., McEvoy, J., Edwinson, A., Stenger, B., Kváč, M., 2013. Human cryptosporidiosis caused by Cryptosporidium tyzzeri and C. parvum isolates presumably transmitted from wild mice. J. Clin. Microbiol. 51, 360-362.
  • Němejc, K., Sak, B., Kvetonová, D., Hanzal, V., Janiszewski, P., Forejtek, P., Rajsky, D., Ravaszová, P., McEvoy, J., Kváč, M., 2013. Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe. Vet. Parasitol. 197, 504-508.
  • Kváč, M., Ondrackova, Z., Květoňová, D., McEvoy, J., Vitovec, J., Rost, M., Sak, B., 2013. The Lesser Egyptian Gerbil (Gerbillus gerbillus) is a suitable host for the long-term propagation of Cryptosporidium andersoni. Exp. Parasitol. 134, 438-442.
  • Kváč, M., McEvoy, J., Loudová, M., Stenger, B., Sak, B., Květoňová, D., Ditrich, O., Rašková, V., Moriarty, E., Rost, M., Macholán, M., Piálek, J., 2013. Coevolution of Cryptosporidium tyzzeri and the house mouse (Mus musculus). Int. J. Parasitol. 43, 805-817.
  • Kváč, M., Kestřánová, M., Pinková, M., Květoňová, D., Kalinová, J., Wagnerová, P., Kotková, M., Vítovec, J., Ditrich, O., McEvoy, J., Stenger, B., Sak, B., 2013. Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa). Vet. Parasitol. 191, 218-227.
  • Kasi, M., Wadhawan, T., Simsek, H., McEvoy, J., Padmanabhan, G., Sletten, D., Khan, E., 2013. Enricher reactor - Permeable reactive biobarrier approach for removing a mixture of contaminants with substrate interactions. Bioresour. Technol. 146, 336-344.
  • Kasi, M., Wadhawan, T., McEvoy, J., Padmanabhan, G., Khan, E., 2013. Effect of carbon source during enrichment on BTEX degradation by anaerobic mixed bacterial cultures. Biodegradation 24, 279-293.

2012

  • Kváč, M., Kestřánová, M., Květoňová, D., Kotková, M., Ortega, Y., McEvoy, J., Sak, B., 2012. Cryptosporidium tyzzeri and Cryptosporidium muris originated from wild West-European house mice (Mus musculus domesticus) and East-European house mice (Mus musculus musculus) are non-infectious for pigs. Exp. Parasitol. 131, 107-110.
  • Herges, G.R., Widmer, G., Clark, M.E., Khan, E., Giddings, C.W., Brewer, M., McEvoy, J.M., 2012. Evidence that Cryptosporidium parvum populations are panmictic and unstructured in the Upper Midwest of the United States. Appl. Environ. Microbiol. 78, 8096-8101.

2011

  • Wadhawan, T., Maruska, Z.B., Siripattanakul, S., Hill, C.B., Gupta, A., Pruess, B.M., McEvoy, J.M., Khan, E., 2011. A new method to determine initial viability of entrapped cells using fluorescent nucleic acid staining. Bioresour. Technol. 102, 1622-1627.
  • Pramanik, S., McEvoy, J., Siripattanakul, S., Khan, E., 2011. Effects of cell entrapment on nucleic acid content and microbial diversity of mixed cultures in biological wastewater treatment. Bioresour. Technol. 102, 3176-3183.
  • Pramanik, S., Khanna, R., Katti, K., McEvoy, J., Khan, E., 2011. Effects of entrapment on nucleic acid content, cell morphology, cell surface property, and stress of pure cultures commonly found in biological wastewater treatment. Appl. Microbiol. Biotechnol. 92, 407-418.
  • Kasi, M., McEvoy, J., Padmanabhan, G., Khan, E., 2011. Groundwater remediation using an enricher reactor-permeable reactive biobarrier for periodically absent contaminants. Water Environ. Res. 83, 603-612.

2010

  • Wadhawan, T., McEvoy, J., Prüß, B.M., Khan, E., 2010. Assessing tetrazolium and ATP assays for rapid in situ viability quantification of bacterial cells entrapped in hydrogel beads. Enzyme Microb. Technol. 47, 166-173.

2009

  • Siripattanakul, S., Wirojanagud, W., McEvoy, J.M., Casey, F.X.M., Khan, E., 2009. A feasibility study of immobilized and free mixed culture bioaugmentation for treating atrazine in infiltrate. J. Hazard. Mater. 168, 1373-1379.
  • Siripattanakul, S., Wirojanagud, W., McEvoy, J.M., Casey, F.X., Khan, E., 2009. Atrazine removal in agricultural infiltrate by bioaugmented polyvinyl alcohol immobilized and free Agrobacterium radiobacter J14a: a sand column study. Chemosphere 74, 308-313.
  • Siripattanakul, S., Wirojanagud, W., McEvoy, J., Limpiyakorn, T., Khan, E., 2009. Atrazine degradation by stable mixed cultures enriched from agricultural soil and their characterization. J. Appl. Microbiol. 106, 986-992.
  • McEvoy, J.M., Giddings, C.W., 2009. Cryptosporidium in commercially produced turkeys on-farm and postslaughter. Lett. Appl. Microbiol. 48, 302-306.
  • Lutgen, E.M., McEvoy, J.M., Sherwood, J.S., Logue, C.M., 2009. Antimicrobial resistance profiling and molecular subtyping of Campylobacter spp. from processed turkey. BMC Microbiol. 9, 203.

2008

  • Siripattanakul, S., Wirojanagud, W., McEvoy, J., Khan, E., 2008. Effect of cell-to-matrix ratio in polyvinyl alcohol immobilized pure and mixed cultures on atrazine degradation. Water Air Soil Pollut: Focus 8, 257-266.
  • Feltus, D.C., Giddings, C.W., Khaitsa, M.L., McEvoy, J.M., 2008. High prevalence of Cryptosporidium bovis and the deer-like genotype in calves compared to mature cows in beef cow-calf operations. Vet. Parasitol. 151, 191-195.

2007

  • Nde, C.W., McEvoy, J.M., Sherwood, J.S., Logue, C.M., 2007. Cross contamination of turkey carcasses by Salmonella species during defeathering. Poult. Sci. 86, 162-167.

2006

  • Feltus, D.C., Giddings, C.W., Schneck, B.L., Monson, T., Warshauer, D., McEvoy, J.M., 2006. Evidence supporting zoonotic transmission of Cryptosporidium spp. in Wisconsin. J. Clin. Microbiol. 44, 4303-4308.
  • Fakhr, M.K., McEvoy, J.M., Sherwood, J.S., Logue, C.M., 2006. Adding a selective enrichment step to the iQ-Check real-time PCR improves the detection of Salmonella in naturally contaminated retail turkey meat products. Lett. Appl. Microbiol. 43, 78-83.

2005

  • Moriarty, E.M., McEvoy, J.M., Lowery, C.J., Thompson, H.P., Finn, M., Sheridan, J.J., Blair, I.S., McDowell, D.A., Duffy, G., 2005. Prevalence and characterisation of Cryptosporidium species in cattle faeces and on beef carcases at slaughter. Vet. Rec. 156, 165-168.
  • Moriarty, E.M., Duffy, G., McEvoy, J.M., Caccio, S., Sheridan, J.J., McDowell, D., Blair, I.S., 2005. The effect of thermal treatments on the viability and infectivity of Cryptosporidium parvum on beef surfaces. J. Appl. Microbiol. 98, 618-623.
  • McEvoy, J.M., Nde, C.W., Sherwood, J.S., Logue, C.M., 2005. An evaluation of sampling methods for the detection of Escherichia coli and Salmonella on turkey carcasses. J. Food Prot. 68, 34-39.
  • McEvoy, J.M., Duffy, G., Moriarty, E.M., Lowery, C.J., Sheridan, J.J., Blair, I.S., McDowell, D.A., 2005. The prevalence and characterisation of Cryptosporidium spp. in beef abattoir water supplies. Water Res. 39, 3697-3703.

2004

  • Moriarty, E.M., McEvoy, J.M., Duffy, G., Sheridan, J.J., Blair, I.S., McDowell, D.A., 2004. Development of a novel method for isolating Cryptosporidium parvum from lean and fat beef and detecting carcass surfaces. Food Microbiol. 21, 275-282.
  • McEvoy, J.M., Sheridan, J.J., McDowell, D.A., 2004. Major pathogens associated with the processing of beef, in: Smulders, F.J.M., Collins, J.D. (Eds.), Safety assurance during food processing. Food safety assurance and veterinary public health Volume 2. Wageningen Academic Publishers, Wageningen, The Netherlands, pp. 57-80.
  • McEvoy, J.M., Sheridan, J.J., Blair, I.S., McDowell, D.A., 2004. Microbial contamination on beef in relation to hygiene assessment based on criteria used in EU Decision 2001/471/EC. Int. J. Food Microbiol. 92, 217-225.
  • McEvoy, J.M., Moriarty, E.M., Duffy, G., Sheridan, J.J., Blair, I.S., McDowell, D.A., 2004. Effect of a commercial freeze/tempering process on the viability of Cryptosporidium parvum oocysts on lean and fat beef trimmings. Meat Science 67, 559-564.

2003

  • McEvoy, J.M., Doherty, A.M., Sheridan, J.J., Thomson-Carter, F.M., Garvey, P., McGuire, L., Blair, I.S., McDowell, D.A., 2003. The prevalence and spread of Escherichia coli O157:H7 at a commercial beef abattoir. J. Appl. Microbiol. 95, 256-266.
  • McEvoy, J.M., Doherty, A.M., Sheridan, J.J., Blair, I.S., McDowell, D.A., 2003. The prevalence of Salmonella spp. in bovine faecal, rumen and carcass samples at a commercial abattoir. J. Appl. Microbiol. 94, 693-700.
  • McEvoy, J.M., Doherty, A.M., Sheridan, J.J., Bailey, D.G., Blair, I.S., McDowell, D.A., 2003. The effects of treating bovine hide with steam at subatmospheric pressure on bacterial numbers and leather quality. Lett. Appl. Microbiol. 37, 344-348.

2001

  • McEvoy, J.M., Doherty, A.M., Sheridan, J.J., Blair, I.S., McDowell, D.A., 2001. Use of steam condensing at subatmospheric pressures to reduce Escherichia coli O157:H7 numbers on bovine hide. J. Food Prot. 64, 1655-1660.

2000

  • McEvoy, J.M., Doherty, A.M., Finnerty, M., Sheridan, J.J., McGuire, L., Blair, I.S., McDowell, D.A., Harrington, D., 2000. The relationship between hide cleanliness and bacterial numbers on beef carcasses at a commercial abattoir. Lett. Appl. Microbiol. 30, 390-395.