Bovine Diarrhea Diagnostic Panel Work-Up (< 4 weeks old)
Common differential list:
- Bacteria: Escherichia coli, Salmonella spp., Clostridium perfringens
- Viruses: Rotavirus, bovine coronavirus, BVDV
- Protozoa: Cryptosporidium parvum
Samples to submit (fresh and fixed):
- Small intestines: duodenum, jejunum, ileum (1-2 segments/each)
- Cecum
- Colon
- Abomasum, rumen
- Kidney
- Liver
- Spleen
- Thymus
- Mesenteric lymph nodes
- Lung
- Adrenal gland
- Any additional gross lesions
Standard tests run at the laboratory (included in the general investigation):
- Histopathology
- Enteric culture
- If E. coli is isolated and significant based on clinical history and histopathology, PCR for virulence factors will be performed
- Bovine diarrhea panel PCR (rotavirus, bovine coronavirus, cryptosporidium parvum)
- Case-by-case basis: depending on age and clinical history
- Clostridium perfringens culture
- If C. perfringens is isolated and significant based on clinical history and histopathology, PCR for toxin genotyping available (additional cost)
Updated 01/10/2025