Johne’s Disease (ELISA)
| Description | Detection of antibody to Mycobacterium avium subsp. paratuberculosis by enzyme-linked immunosorbent assay (ELISA). |
|---|---|
| Department | Serology |
| Fee | $8 |
| Days Tested | Wed | Fri (Oct 1 - May 31), Wed (June 1 - Sept 30) |
| Turnaround Time | 1-2 days |
| Species | Bovine, Caprine, Ovine |
| Specimen | Serum or milk (1 ml minimum) |
| Sampling Requirements | Collect blood in a serum separator (SST) or red top tube (RTT). For best sample quality and to minimize hemolysis during transport, serum should be transferred off the clot to a new sterile tube with no additives. |
| Collection Container | Serum separator tube (SST) or red top tube (RTT). |
| Packaging Instructions |
Label all samples with unique identifiers. For multiple samples, also number the tubes consecutively. Remove needles from tubes prior to shipment. Ensure caps are fully seated and tubes are protected against breakage. |
| Additional Information |
APHIS-approved lab for Import/Export. Results are reported as S/P ratio (Sample/Positive) not a titer. This test is not currently approved by USDA for Ovine testing and results are reported with a disclaimer. Contact the laboratory prior to submitting large numbers of samples (>100 samples) to ensure sufficient reagents are available. Note: Samples must be received during business hours the day before scheduled testing to ensure inclusion in the test run. If not, the sample will be tested on the following scheduled test day. STAT testing may be available, contact the lab for more information. |