Tritrichomonas foetus Collection and Submission Guide

About the Test

The NDSU-VDL uses a PCR assay to test for Tritrichomonas foetus.
The test is sensitive and does not cross-react with commensals or fecal trichomonads.
The best results are obtained from properly collected and transported samples.

Quantity: >2 mLs
Preferred Container: Sterile, plastic screwcap (10-50 mL Falcon tube) or plastic red top tube
Avoid: Glass tubes, Whirl-Paks, and urine cups. Broken, wet or leaking samples are a safety hazard and contamination concern and may not be tested.
Assay is not approved for samples submitted in InPouches, serum separator tubes, tubes with gel or other preservatives (i.e. EDTA) & WILL NOT be tested.
Preputial wash samples should be collected by a veterinarian, using a clean pair of exam gloves for each sample to avoid cross contamination.
Remove excess material, soiled hair, fecal flakes, dirt, etc. that lead to false negatives
The VDL may reject or report with a disclaimer if sample is contaminated
DO NOT cleanse the sampling area with disinfectants as those could inactivate the parasite
A proper sample will be slightly cloudy with visible smegma.

Type animal IDs into the Herd Serology/PCR Submission form and email to the VDL
Enclose a copy with the samples
Include the collection date on the submission form. Samples received >5 days after collection are more likely to produce false negative results.
Indicate whether testing should be individual or pooled. If not specified, testing may be delayed and will be determined by the VDL.

Samples must be received by the VDL within 5 days of collection date. Samples that are received more than 5 days old will be tested at the VDL’s discretion and reported with a disclaimer.
Ship samples overnight with cold packs or drop-off in the 24hr cooler.
If sending in glass red top tubes, we highly recommend individually wrapping in paper towels or napkins and placing in individual baggies to avoid breaking and leaking on other samples.
Avoid shipping on Fridays, during expected bad weather, long holiday weekends, or expected closures. The NDSU VDL does not receive and process samples on weekends or University/State closures.

For pooled testing, the laboratory performs the pooling to ensure equal animal representation and traceability.
Positive/detected pools will automatically be tested individually. Additional charges apply.
Pooled testing is not recommended for herds with known or suspected Tritichomonas.
The Submitter must verify destination-state requirements for individual vs. pooled testing.
If Campylobacter testing is requested, samples cannot be pooled.

Revised 09-2026