Histology Core Facility

Histology Core Facility

The Histology Core is located in Sudro 134 in the department of Pharmaceutical Sciences.

Sample submission is Monday – Friday, 1:00pm-3:00pm or by appointment

Contact
Gwendolyn Thomas
Histology Core Director

Services


Tissue Preparation

  • All tissues submitted to the core must be grossed, placed in a cassette and fixed for a minimum of 24 hours, up to 48 hours, prior to submission.
    • Fixation: all tissues must be fixed prior to submission, preferably in 10% Neutral Buffered Formalin
    • Submission: all samples will be submitted in 70% ethanol
  • Tissue processing; dehydration, clearing, infiltration, embedding, slide preparation and scanning.

Microtomy

  • Paraffin microtomy

Histostaining

Service will include protocols, and advice on tissue processing, embedding orientation, and routine or special staining. Paraffin embedding, processing, sectioning and a staining service that will include:

  • Hematoxylin & Eosin (H&E): general tissue staining
  • Other special stains may be available on an individual basis or will be supplied by the investigator.

Whole Sliding Scanning

Scanned images will be stored for 45 days from the day of project completed notification. The core will work with investigators to transfer data once available. After 45 days, the core is not responsible for the storage of data due to limited space.

  • Diagnostic quality, high-throughput resolution images at 20X or 40X magnification

Publication Acknowledgment

Acknowledgments can be referenced as: Histological services were provided by the NDSU Histology Core Facility supported by the Center for Diagnostic and Therapeutic Strategies in Pancreatic Cancer funds are supported through NIH award number (2 P20GM 109024).

Center for Diagnostic and Therapeutic Strategies in Pancreatic Cancer